https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Physicochemical properties of Gac (Momordica cochinchinensis (Lour.) Spreng) seeds and their oil extracted by supercritical carbon dioxide and soxhlet methods https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34879 2) and hexane (Soxhlet). The Gac seed kernel accounted for 66.4 ± 2.7% of the seed weight, and 53.02 ± 1.27% of it was oil. The SC-CO2 oil had a higher quality than the Soxhlet oil for important criteria, such as peroxide (0.12 ± 0.02 vs. 1.80 ± 0.01 meq O2/kg oil), free fatty acid (1.74 ± 0.12 vs. 2.47 ± 0.09 mg KOH/g oil) and unsaponifiable matter (33.2 ± 1.5 vs. 52.6 ± 2.4 g/kg) values, respectively. It also had a better colour (light yellow vs. dark greenish brown) and a higher antioxidant capacity measured with the DPPH (52.69 ± 0.06 vs. 42.98 ± 0.02 µmol Trolox equivalent/g oil) and ABTS (2.10 ± 0.12 vs. 1.52 ± 0.06 µmol Trolox equivalent/g oil) assays. However, a higher yield (53.02 ± 1.27 vs. 34.1 ± 0.8%) was obtained for the Soxhlet oil. Unless refined, the oils would not be edible due to their high unsaponifiable matter, but the SC-CO2 oil would need less refining. Alternatively, the high unsaponifiable matter in the oil, especially in the Soxhlet oil, may make it useful for medicinal purposes.]]> Tue 03 Sep 2019 18:18:23 AEST ]]> Improving the vanillin-sulphuric acid method for quantifying total saponins https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34881 Momordica cochinchinensis Spreng) seed kernels. To improve the method, a solvent evaporation step was added prior to the colorisation reaction to prevent undesired solvent interference during the reaction step. Using this modified protocol for the aescin standard curve and the Gac seed kernel extract eliminated any solvent interference. Thus, this improved protocol is recommended for the quantification of the saponin content of plant extracts irrespective of which extraction solvent is used.]]> Tue 03 Sep 2019 18:09:42 AEST ]]> Effect of solvents and extraction methods on recovery of bioactive compounds from defatted Gac (Momordica cochinchinensis Spreng.) seeds https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34880 −1) while water-saturated n-butanol and methanol extracts were characterized by their highest content of saponins (40.75 ± 0.31 and 38.80 ± 2.82 mg AE g−1, respectively). Aqueous extract with microwave assistance achieved the highest phenolics (3.18 ± 0.04 mg GAE g−1). As a measure of antioxidant capacity, the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assay gave highest value to the aqueous microwave extract (23.56 ± 0.82 μmol TE g−1) while the ferric reducing antioxidant power (FRAP) assay gave highest values to water-saturated n-butanol and 70% ethanol extracts (5.25 ± 0.04 and 4.71 ± 0.39 μmol TE g−1, respectively). The total solids value was highest using water with microwave assistance (141.5 g kg−1) while ultrasound treatment did not improve any extractions. Therefore, trypsin inhibitors are suitably recovered using water while water-saturated n-butanol or methanol is for saponins, both using a conventional method. Microwave extraction is suitable for phenolics recovery. These conditions are recommended for an efficient recovery of bioactive compounds from defatted Gac seeds.]]> Tue 03 Sep 2019 18:08:26 AEST ]]> Bioactive composition, antioxidant activity, and anticancer potential of freeze-dried extracts from defatted Gac (Momordica cochinchinensis Spreng) seeds https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34442 Tue 03 Sep 2019 18:00:55 AEST ]]> Optimisation of the microwave-assisted ethanol extraction of saponins from Gac (Momordica cochinchinensis Spreng.) seeds https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34443 Tue 03 Sep 2019 18:00:49 AEST ]]> Optimised extraction of trypsin inhibitors from defatted gac (Momordica cochinchinensis spreng) seeds for production of a trypsin inhibitor-enriched freeze dried powder https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37414 Thu 12 Nov 2020 17:17:51 AEDT ]]> Investigation of the most suitable conditions for dehydration of Tuckeroo (Cupaniopsis anacardioides) fruits https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40415 Cupaniopsis anacardioides) is an Australian native tree, possessing high level bioactivity and antioxidant activity. To prevent deterioration of active constituents, appropriate drying practices must be determined. This study comparatively evaluates the impact of a range of drying methods including freeze-, microwave-, vacuum-, hot air- and sun-drying on the physical, phytochemical and antioxidant characteristics of Tuckeroo fruit. Experimental results showed that the five drying methods had significant impact on the physicochemical properties and antioxidant activity of the fruits. Of the drying methods assessed, freeze drying best preserved Tuckeroo activity, recording higher total phenolic content (TPC) (81.88 mg gallic acid equivalent (GAE)/g), total flavonoids (TFC) (107.71 mg catechin equivalent (CAE)/g), proanthocyanidins (TPro) (83.86 mg CAE/g) and exhibited the strongest antioxidant capacity. However, vacuum drying at 65 kPa, 100 ℃ for 5 h is recommended for drying Tuckeroo fruits for further processing in a large scale as it also retained high levels of TPC, TFC and TPro (58 mg GAE/g, 91 mg CAE/g and 74 mg CAE/g, respectively).]]> Mon 11 Jul 2022 14:44:41 AEST ]]>